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 | Volume 14, Number 3 (Fall 2010 2010) |  | |
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 | Alterations of the expression of RGS4 and RGS10 proteins in the anticonvulsant effects of low frequency stimulation on perforant path kindling in adult male rats |  |
| | Author(s): Simin Namvar, Javad Mirnajafi-Zadeh , Mohammad Javan and Maryam Zeraati | | | Study Type: Original Research | Subject: Nervous system | | | Article abstract: | | Introduction: Application of low-frequency stimulation (LFS) is a new method for treatment of drug resistant
epileptic patients. Previous studies demonstrated that activation of receptors coupled to Gi proteins is one of the
mechanisms of the anticonvulsant effect of LFS. Thus, in this study, alterations in the expression of RGS4 and RGS10
proteins, as negative regulators of Gi proteins, were investigated.
Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner (12 stimulation per day, 1
ms pulse duration at 50 Hz). LFS (8 stimulation per day, 0.1 ms pulse duration at 1 Hz, 200 pulses) was applied to the
perforant path 5 minute after the termination of kindling stimulations. After electrophysiological recordings for 6 days,
the dentate gyrus of the animals was removed and RGS4 and RGS10 protein expression was studied by western blotting
technique.
Results: Application of LFS significantly retarded kindling acquisition and increased the number of stimulations to
achieve different stages of seizure. LFS also significantly reduced after discharge duration. In addition, application of
LFS after kindling stimulation reduced the expression of RGS4 and RGS10 proteins.
Conclusion: Results of the present study showed that LFS has anticonvulsant effects on the perforant path kindling.
Application of LFS following kindling stimulation reduced the expression of RGS4 and RGS10 proteins. Reduction of
the expression of these proteins results in the longer activation of signaling pathways of Gi proteins, which may be
responsible for LFS anticonvulsant effects.
| | | Keywords: Seizure, Low-frequency stimulation, RGS proteins, Dentate gyrus, Kindling, | |
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Send citation data to: CiteULike | RefWorks | | Namvar Simin, Mirnajafi-Zadeh Javad, Javan Mohammad, Zeraati Maryam, Alterations of the expression of RGS4 and RGS10 proteins in the anticonvulsant effects of low frequency stimulation on perforant path kindling in adult male rats Physiology and Pharmacology, 2010; 14 (3) :234-241 URL http://www.phypha.ir/ppj/browse.php?a_code=A-10-51-12&slc_lang=en&sid=1 | | |
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